Figure 2

Allografts from donor-derived MDSCs treated recipients exhibit ameliorated T cell mediated inflammation and increased recipient-derived endogenous MDSCs. (A) Representative example of the allograft histology (H&E staining) from the MDSCs treated group and the cMDCs treated control on POD7. Scale bar represent 500 μm (left) and 100 μm (right). Data represents the one of 3 separate experiments. (B) Allografts were harvested on POD7 and stained with anti-CD3, anti-CD11b (green) and DAPI (blue). Scale bars represent 200 μm. Positive cells were qualitied by ImageJ and expressed as mean ± SEM. ****p < 0.0001, two-tailed unpaired t test. (C) Flow cytometry analysis of BALB/c MDSCs treated C57BL/6 recipients’ graft infiltration lymphocytes (GILs) on POD7. Graphs showing significant decrease of the proportion of CD44⁺CD62L^lo and Ki67⁺ in CD4⁺FoxP3^- T cells in the MDSCs treated group. (D) qRT-PCR analysis of allografts (whole tissues) on POD3 (n = 3 per group). Graph represented as RQ (relative quantification) = 2^-ΔΔCt. Naïve BALB/c heart serve as basic ΔCt (n = 3, not shown in graph). Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, two-tailed unpaired t test.