Figure 7
From: Tolerance to alkaline ambient pH in Aspergillus nidulans depends on the activity of ENA proteins
Constitutive expression of enaA under the control of the gpdAmini promoter. (A) Expression, in a wild-type background and under inducing (+) or non-inducing (−) conditions, of enaA driven by the native or the gpdAmini promoters. Strains that integrated one or two copies of the gpdAmini::enaA plasmid were analyzed. Ribosomal RNA is shown as a loading control. (B) Same experiment as in (A) but carried out with samples corresponding to the palA1 mutant background. (C) Phenotypes of wild-type (left block of images) or palA1 (right) strains bearing zero (–), one (1 ×) or two (2 ×) copies of the gpdAmini::enaA plasmid after 48 h of culture at 37 °C on AMM (row 1), AMM with LiCl (0.3 M; row 2), KCl (1 M; row 3), NaCl (1 M; row 4) or neomycin (2 mg/mL; row 6), and AMM adjusted to pH 8 (row 5).
