Figure 2 | Scientific Reports

Figure 2

From: Ischemia/reperfusion injured intestinal epithelial cells cause cortical neuron death by releasing exosomal microRNAs associated with apoptosis, necroptosis, and pyroptosis

Figure 2The alternative text for this image may have been generated using AI.

(A) Representative immunofluorescence images showing colocalization of MAP2 (neuron-specific nuclear protein marker, red) and synaptophysin (synaptic vesicle protein marker, green) in cortical cells in each experimental group. (B) Representative immunofluorescence photomicrographs of MAP2 (red) and TUNEL (apoptosis marker, green) positive cells in each experimental group. The DNase-treated neuron was used as a positive control. Neurons were not treated with TdT as a negative control. All cell nuclei were counterstained with DAPI (blue). (C) Quantification of synaptophysin in neurons were evaluated by the ratio of MAP2 and synaptophysin double-positive cells (yellow) to DAPI positive cells (blue). Percentages of apoptotic neurons were evaluated by the ratio of TUNEL and MAP2 double-positive cells (yellow) to DAPI positive cells (blue). Quantification of (D) MAP2 and synaptophysin staining and (E) MAP2 and TUNEL staining fluorescence intensity from each group were normalized to an average intensity of the Neuron control+37 °C group. Each bar represents the means ± S.D. of 6 independent cultures in each experimental condition. *p < 0.05, OGD-IEC-6+Neuron+37 °C vs. Control Neuron+37 °C or IEC-6+Neuron+37 °C; +p < 0.05, OGD-IEC-6+Neuron+37 °C. Scale bar, 100 μm.

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