Figure 1
Effects of LIPUS on H2O2-induced cell viability inhibition and inflammatory signals in renal tubule NRK52E cells. Cells were treated with H2O2 (25 μM) for 24 h with or without LIPUS (30 or 100 mW/cm2) treatment. LIPUS was performed to the cell culture for a period of 15 min before the beginning of the experiment (H2O2). (A) The cell viability was determined by MTT assay. Both 30 and 100 mW/cm2 LIPUS 30 were performed. (B) The 100 mW/cm2 LIPUS was performed. The protein levels of inducible NO synthase (iNOS), cyclooxygenase-2 (Cox-2), phosphorylated NFκB-p65 (p-p65), and p65 were determined by Western blotting. Data are presented as means ± SEM for three to five independent experiments. *p < 0.05 versus control. #p < 0.05 versus H2O2 alone.
