Figure 3 | Scientific Reports

Figure 3

From: Impaired brain homeostasis and neurogenesis in diet-induced overweight zebrafish: a preventive role from A. borbonica extract

Figure 3

DIO increases BBB leakage and neuroinflammation. (A) Dorsal view pictures of the CTRL and DIO zebrafish brains following Evans blue staining. Note the blue staining observed in DIO-treated fish compared to controls (n = 6 brains). (B–E) qPCR gene expression analysis of nfkb and pro-inflammatory cytokines (il1β, il6 and tnfα) in CTRL and DIO fish (n = 3 pools of 2 brains). (F) Couting of ramified and amoeboid microglia in the ventral telencephalon (Vv Vd) and the anterior hypothalamus (Hv) regions in both CTRL and DIO-treated fish. (G) Representative L-plastin immunohistochemistry pictures showing microglia morphology in CTRL and DIO zebrafish brain in the subpallium (Vv Vd). Note the apparent increase in amoeboid-like morphology and the stronger L-plastin staining intensity in DIO fish compared to the control ones. n = number of fish. Student's t-test: * p < 0.05; **p < 0.01; ***p < 0.001. Error bar: standard error of the mean (SEM). Scale bar: 0.8 mm (A); 148 μm (G).

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