Figure 1

Transgenic tobacco lines expressing GhSAMDC1 (WT, 3–1, 3–2, 4–3 and 4–4 means wild type and transgenic lines 3–1, 3–2, 4–3 and 4–4. Leaves from three 30 days old plants were sampled for polyamine detection and expression analysis. Data are means of 3 replicates ± SE. Bars labeled with * and ** are significantly different at P < 0.05 and 0.01, respectively. WT as control for significance analysis). (a) Expression vector of GhSAMDC1; (b) q-RT PCR analysis of GhSAMDC1; (c–f) total polyamines, putrescine, spermidine, spermine levels; (g) q-RT PCR analysis of NtSPDS1- 5 and NtSPMS.