Figure 3

Normal function of the group 1 mGluR and voltage-dependent Ca²⁺ channels in ShcB-KO PCs. (A) Representative traces of PF-EPSCs recorded from WT and ShcB-KO (KO) PCs in response to paired-pulse stimuli (interpulse interval: 50 ms) before (DHPG (−)) and after (DHPG (+)) bath application of 50 μM DHPG, a specific agonist of the group 1 mGluR. PF-EPSC: parallel fiber-excitatory postsynaptic current, PC: Purkinje cell, DHPG: (S)-dihydroxyphenylglycine, mGluR: metabotropic glutamate receptor. (B, C) Decrease in (B) PF-EPSC amplitude by application of DHPG, accompanied by increase in (C) paired-pulse ratio. Amplitudes are normalized to the average before DHPG application, and are shown as mean ± s.e.m. for WT (n = 10) and ShcB-KO (KO; n = 9) mice. (D–H) Ca²⁺-influx through voltage-dependent Ca²⁺ channel is normal in ShcB-KO Purkinje cells (PCs). (D, E) Ca²⁺-transients elicited by depolarization-pulse (from − 80 to 0 mV for 1 s) to PCs in WT cerebellum. The response was inhibited by removing Ca²⁺ from extracellular fluid (0 Ca²⁺) or adding 200 nM ω-agatoxin (Aga). (D) representative image, (E) averaged values of amplitude. (F–H) Ca²⁺ influx induced by depolarization pulse to WT or ShcB-KO PCs. (F) Representative image, (G) averaged values of amplitude and (H) half decay time (τ) of Ca²⁺ influx of WT (n = 7) and ShcB-KO (KO; n = 7) mice. Values are normalized to those before depolarization.