Figure 3
From: 3D-bioprinted all-inclusive bioanalytical platforms for cell studies
(A) Schematics of printed Pluronic molds and resulting (B) PDMS casts for concave wells and channels. (C) Image of printed Pluronic molds used to fabricate PDMS concave wells and channels (scale bar: 2 mm). The process of (D–E) 3D printing GelMA-HCT116 structures within concave wells, (F–G) assembling the microfluidic platform, and (H) media perfusion of GelMA-HCT116 structures. (I) Photograph of the concave well-based microfluidic platform (scale bar: 2 mm). (J–L) Image representatives of three different toroidal formed structures of the 3D-bioprinted GelMA and HCT116 cell mixture. Live HCT 116 cells within the constructs were labeled with Calcein AM. Image representatives show the toroidal GelMA and HCT116 constructs with (J) smaller, (K) larger inner cavity, and (L) small cell island formed within the inner cavity of the ring.
