Figure 1

Effects of FASN inhibitor G28UCM on growth and lipid content of SKOV3 and OVCAR3 cells. (a) The bipartite pattern of relative growth sensitivity of SKOV3 and OVCAR3 cells against G28UCM. Cells were exposed for 48 h and 72 h to various concentrations of the drug prior to cell growth measurement using a formazan dye assay. At low doses both cell lines are equally sensitive, while at doses ≥ 20 µM, OVCAR3 appears more sensitive than SKOV3. Means ± SD, n = 3. Two-tailed Student's t-test, p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***) between SKOV3 and OVCAR3 treated cells. (b) TLC separation and changes of the major cellular lipid classes in SKOV3 and OVCAR3 cells upon G28UCM treatment (20 µM, 72 h). Relative changes of the different phospholipid classes in (c) SKOV3 and (d) OVCAR3 treated with 0.1% DMSO (Control) or 40 µM G28UCM for 8 h and 24 h. Values are sums of signal intensity of lipid species relative to class specific internal standards added to the samples before analysis (Ratio vs. Int. Std.) (Supplemental Figure S2). CE cholesterol esters, CL cardiolipin, DAG diacylglycerols, LPC lysophosphatidylcholine, PC phosphatidylcholine, PE phosphatidylethanolamine, PG phosphatidylglycerol, PI phosphatidylinositol, PL phospholipids, PS phosphatidylserine, SM sphingomyelin, TAG triacylglycerols.