Figure 2 | Scientific Reports

Figure 2

From: Membrane disruption, but not metabolic rewiring, is the key mechanism of anticancer-action of FASN-inhibitors: a multi-omics analysis in ovarian cancer

Figure 2

Effects of the FASN inhibitor G28UCM on the phosphatidylcholine (PC) composition of SKOV3 and OVCAR3 cells. Changes in the relative composition of PC species containing PUFAs with > 2 total double bonds (DBs) in (a) SKOV3 and (b) OVCAR3 cells treated with 0.1% DMSO and 40 µM G28UCM for 8 h and 24 h. Displayed is the relative composition of PC species with > 2 DBs in % of total PC (dashed lines). Values are means ± SD (n = 3). Dashed lines indicate the PC species mostly affected by FASN-inhibition. Letter code of the PUFAs: A, arachidonate (20:4); E, eicosapentaenoate (20:5); P, palmitate (16:0); S, stearate (18:0). (c) Schematic view of the major biosynthesis pathways of very long-chain polyunsaturated FAs (PUFAs) derived from essential ω-3 and ω-6 FAs. Boxes indicate those PUFAs, which were found to be mostly affected by FASN-inhibition.

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