Figure 3 | Scientific Reports

Figure 3

From: Blood collection technique, anticoagulants and storing temperature have minor effects on the isolation of polymorphonuclear neutrophils

Figure 3

Effects of storing time and blood collection technique on PMN respiratory burst activity: Fluorescence as indicator of accumulation of reactive oxygen species (oxidative burst) was determined, depicted is the mean over 1,000 cells counted. (A) Respiratory burst activity depicted for all methods 0, 1, 3 and 7 h after the isolation process. (B) Respiratory burst activity for different storing times drawn with butterfly cannula in free flow. (C) Respiratory burst activity for different storing times drawn with butterfly cannula and syringe. (D) Respiratory burst activity for different storing times drawn with vacuum cannula. (E) Respiratory burst activity for different storing times drawn with vein catheter in free flow. (F) Respiratory burst activity for different storing times drawn with vein catheter with syringe. *p < 0.05; **p < 0.01, ***p < 0.001, BF-FF and V-V n = 10, BF-S, VC-FF and VC-S n = 9, data are shown as mean ± SEM.

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