Figure 4 | Scientific Reports

Figure 4

From: Blood collection technique, anticoagulants and storing temperature have minor effects on the isolation of polymorphonuclear neutrophils

Figure 4

Effects of storing time and blood collection technique on PMN burst—potential of activation: Depicted is the difference between the mean fluorescence of the activated sample and the mean fluorescence of the control sample. Fluorescence was used as indicator of accumulation of reactive oxygen species (oxidative burst). (A) Δ Burst depicted for all methods 0, 1, 3 and 7 h after the isolation process. (B) Δ Burst for different storing times drawn with butterfly cannula in free flow. (C) Δ Burst for different storing times drawn with butterfly cannula and syringe. (D) Δ Burst for different storing times drawn with vacuum cannula. (E) Δ Burst for different storing times drawn with vein catheter in free flow. (F) Δ Burst for different storing times drawn with vein catheter with syringe. *p < 0.05; **p < 0.01; ***p < 0.001, BF-FF and V-V n = 10, BF-S, VC-FF and VC-S n = 9, data are shown as mean ± SEM.

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