Figure 6 | Scientific Reports

Figure 6

From: Blood collection technique, anticoagulants and storing temperature have minor effects on the isolation of polymorphonuclear neutrophils

Figure 6

Effects of anticoagulant for different storing temperatures after isolation: blood samples were mixed with EDTA, heparin or sodium citrate. (A) PMN counts. (B) respiratory burst activity, determined by fluorescence as mean over 1,000 cells counted. (C) Δ Burst as difference between the mean fluorescence of the activated sample and the mean fluorescence of the control sample. No significant differences between the anticoagulants were found. n = 6 except for the timepoint ‘before’ (n = 3), data are shown as mean ± SEM.

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