Figure 2
From: An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA
SG7 is an inhibitor of mouse and human VISTA. (a) Mouse VISTA-Fc binding to activated mouse T cells at pH 6.0 with increasing concentrations of pre-complexed SG7 or isotype control. (b) Human VISTA-Fc binding to activated human T cells at pH 6.0 with increasing concentrations of pre-complexed SG7 or isotype control. (c) Rescuing effect of SG7 and VSTB112 on the activation of Jurkat NFAT (BFP) T cells in the presence of human VISTA-Fc. P-values obtained by one-way ANOVA (Tukey’s multiple comparison test), ***p < .005, ****p < .0005. (d) Competition ELISA to test simultaneous binding of SG7 and microtiter well-coated BMS767 or VSTB112 to soluble human VISTA. Binding signal of SG7 bound to the complex of VISTA and coated antibody is shown with increasing concentrations of SG7. In this assay, only the anti-His positive control can bind simultaneously to SG7 and VISTA. Mean ± SD for triplicate measurements are shown for all panels.
