Figure 1

Mouse hearts 2 d after MI are infiltrated in pro-inflammatory immune cells. Cell suspensions of enzymatically digested hearts from sham and MI mice were stained with fluorescently labeled antibodies. Representative dot plots identified: (A) neutrophils as CD11b^posLy6G^pos cells, which were stratified into N1 (CD206^neg) and N2 (CD206^pos) subpopulations; (B) monocytes Lin^negCD11b^pos (F4/80/CD11c/MHCII)^low with Ly6C^high and Ly6C^low expression; (C) macrophages Lin^negCD11b^posF4/80^high with Ly6C^high and Ly6C^low expression. Numerical representations of neutrophils (D), monocytes (E), and macrophages (F) and their subpopulations per mg of tissue; pie chart represents contribution of pro-inflammatory and anti-inflammatory cell subpopulations. Number of cells shown as mean; sham n = 3, MI n = 4; *P < 0.05; **P < 0.01 (unpaired t test). Quantitative PCR on mRNA isolated from hearts 2 d after sham and MI. Messenger RNA expression of pro-inflammatory (G) and anti-inflammatory (H) cytokines and chemokines in whole hearts 2 d after sham and MI. Data are means ± SEM; sham n = 4, MI = 4; *P < 0.05; **P < 0.01; ***P < 0.001 (unpaired t test).