Figure 2
Effects of CMCs on neutrophils in vitro and in vivo. CMCs (5 × 105) were injected in the mouse hearts 2 d after MI. Seven days later, hearts were harvested and neutrophils were detected with flow cytometry (A), and quantified (B). Number of cells shown as mean; sham n = 5, MI = 6; **P < 0.01 (unpaired t test). Bone marrow neutrophils were subjected to migration assay with modified Boyden chamber plates to CMC conditioned media. Number of migrated cells shown as mean ± SEM; n = 5; *P < 0.05; ***P < 0.001; ****P < 0.0001 (one-way ANOVA) (C). Bone marrow neutrophils were cultured in serum depleted media for 24 h in the presence or absence of CMCs conditioned media. Apoptotic cells were detected with Annexin V/PI staining (D) and quantified with flow cytometry (E). Number of apoptotic cell shown as mean ± SEM; n = 5; **P < 0.01; ****P < 0.0001 (multiple t test).
