Figure 4

CMC secretome increases monocyte migration and macrophage proliferation, phagocytosis, and inhibits pro-inflammatory activation in vitro. Bone marrow monocytes were subjected to migration assay with modified Boyden chamber plates to CMC conditioned media. Number of migrated cells shown as mean ± SEM; n = 4; ****P < 0.0001 (one-way ANOVA) (A). Naïve bone marrow macrophages (M0) were stimulated with LPS (100 ng/mL) and IFNγ (20 ng/mL) (M1), or IL-4 (10 ng/mL) and IL-13 (10 ng/mL) in the precence CMC conditioned media or control media. After 24 h macrophage number was evaluated with microscopy (B); phagocytosis of FITC IgG opsonized latex beads was determined with flow cytometry (C), and expression of pro-inflammatory gene expression was quantified with qPCR (D). Data are means ± SEM; n = 6; *P < 0.05; **P < 0.01; ****P < 0.0001 (one-way ANOVA).