Figure. 6

CMCs secrete chemokines for neutrophils and monocytes. Expression of CC (A) and CXC chemokines (B) was determined with RNAseq. Expression of CC and CXC chemokines in CMC conditioned media was evaluated with Chemokine Array (C). Bone marrow cells were subjected to migration assay with modified Boyden chamber plates to CMC conditioned media in the presence of AMD3100, SD252002, or CCR2 inhibitor (D). Number of migrated cells shown as mean ± SEM; n = 4; *P < 0.05; ***P < 0.001; ****P < 0.0001 (one-way ANOVA). Schematic of chemokine-chemokine receptor interaction (E).