Figure 9

M101 reduces P. gingivalis growth in vitro. M101 treatment (1 g/L) decreased P. gingivalis (Pg) biofilm on glass surface; (A,B) scanning Electron Microscopy at 2 h (SEM) of (A) Pg biofilm, (B) M101-treated Pg biofilm (C,D) live/dead assay of Pg biofilm at 6 h (green = living Pg, red = dead Pg), (E) relative mRNA expression of Pg in Pg biofilm treated with M101 (1 g/L) for 6 h by RTqPCR, (F,G) live/dead assay of Pg biofilm at 24 h (green = living Pg, red = dead Pg), (H) relative mRNA expression of Pg in Pg biofilm treated with M101 (1 g/L) for 24 h by RTqPCR. Data are expressed as the mean ± SD. *Difference versus untreated Pg, p < 0.05, †difference versus alcohol 75%, p < 0.05.