Figure 3 | Scientific Reports

Figure 3

From: Differentiated ovine tracheal epithelial cells support the colonisation of pathogenic and non-pathogenic strains of Mannheimia haemolytica

Figure 3

Immunofluorescence microscopy of PH278- and PH62-infected differentiated OTECs. Differentiated OTECs were infected with either pathogenic (PH278) or non-pathogenic (PH62) M. haemolytica isolates for the indicated periods of time. Non-adherent bacteria were removed by washing and tissues were fixed in paraformaldehyde. Following permeabilisation, bacteria were immunostained using an anti-M. haemolytica whole-cell antibody (green). Host cell cytoskeleton and cilia were stained with anti-β-tubulin (red) and nuclei were stained with DAPI (blue). Samples were mounted and viewed by fluorescence microscopy. (A) Wide-field microscopy images of infected OTECs at various time-points post-infection. These demonstrate the formation of invasive foci of PH278 and various stages in the formation of PH62 microcolonies. (B) Orthogonal representation of a confocal stack from a PH278-infected culture at 24 h post-infection showing the three-dimensional rounding and sloughing of cells surrounding an invasive focus of infection.

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