Table 2 Phenotypic characterisics of mecC-MRSA.

ID	CC	agr	CS	Haemolysis on SBA		BF	agr acitivity level (predicted)
ID	CC	agr	CS	CAMP	SPDH	BF	agr acitivity level (predicted)
IMT31818	599	I	−	−	None	0	0
IMT32509	599	I	+	+	α, β, δ	0	++
IMT32513	599	I	+	+	α, β, δ	0	++
IMT36943	599	I	+	+	α, β, δ	0	++
IMT36947	599	I	+	+	α, β, δ	1	++
IMT36948	599	I	+	+	α, β, δ	1	++
IMT38116	599	I	+	+	α, β, δ	0	++
IMT39824	599	I	−	−	None	0	0
IMT39825	599	I	+	+	α, β, δ	0	++
RKI5962	599	I	+	+	α, β, δ	0	++
RKI5963	599	I	+	+	α, β, δ	0	++
RKI5964	599	I	+	+	α, β, δ	0	++
IMT36945	49	II	−	−	None	0	0
RKI5972	49	II	+	+	α, β, δ	0	++
IMT31819*	130	III	−	−	None	0	0
IMT32510	130	III	+	Weak +	Weak β	0	++
IMT32929	130	III	+	Weak +	β	1	++
IMT34478	130	III	+	−	None	1	0
IMT34479	130	III	+	+	β, δ	1	++
IMT34480	130	III	+	+	β, δ	1	++
IMT34488	130	III	+	+	α, β, δ	0	++
IMT34489	130	III	−	−	None	0	0
IMT34491	130	III	+	+	α, β, δ	0	++
IMT36946	130	III	+	+	α, β, δ	0	++
IMT36950	130	III	+	+	α, β, δ	0	++
IMT38115	130	III	+	+	α, β, δ	0	++
IMT38119*	130	III	+	+	α, β, δ	0	++
IMT39816	130	III	+	+	α, β, δ	0	++
IMT39819	130	III	+	+	α, β, δ	2	++
IMT40506	130	III	+	+	α, β, δ	1	++
IMT41554	130	III	+	+	α, β, δ	1	++
RKI5965	130	III	Weak +	+	α, β, δ	1	++
RKI5966*	130	III	−	+	β	0	0
RKI5967	130	III	+	+	α, β, δ	0	++
RKI5968	130	III	+	+	α, β, δ	1	++
IMT34485	1943	IV	+	+	α, β, δ	0	++
IMT36952	1943	IV	−	−	None	2	0
IMT38113	1943	IV	+	−	α, δ	0	++
IMT39820	1943	IV	+	+	α, β, δ	0	++
IMT40504	1943	IV	+	+	α, β, δ	0	++
IMT40507	1943	IV	+	−	α, δ	0	++
RKI5969	1943	IV	+	+	α, β, δ	0	++
RKI5970	1943	IV	+	+	α, β, δ	0	++
RKI5971	1943	IV	+	+	α, β, δ	0	++
RKI5973	1943	IV	Weak +	−	α, δ	2	++

Genomic variation within the agr encoding region is indicated by use of bold ID letters (for details see Fig. 2). The isolates further investigated with respect to biofilm structures using CLSM are marked with *. Agr activity prediction according to Hld values presented in Fig. 4a (0, +, ++).
ID, isolate number; CC, clonal complex; agr, accessory gene regulatory type; CS, colony spreading (phenotypical verification for agr functionality); CAMP, phenotypical verification for β-haemolysin production; SPDH, test results for synergistic production of different heamolysins using RN4220 (phenotypical verification of α-, β- and δ-haemolysin production); BF, biofilm formation (mean absorbance 492 nm; values: 0, < 0.2811; 1, 0.2811 < x < 1.0; 2, > 1.0); + , positive; −, negative.

Search