Figure 7
From: 3D histopathology of human tumours by fast clearing and ultramicroscopy
Comparison of tissue compounds preservation of specimen processed with pathoDISCO and standard histology. (a) Cleared sample labelled with histo-marker. (b) Post-clearing paraffin-embedded sample with 4-dots labelling. Arrows showing the sites of 4-dots labelling; (c) whole specimen slice, proceeded with standard FFPE/H&E; (e) zoom-in, showing the loss of intra-ductal cellular mass due to the mechanical tissue processing. (d,f,g) Several optical planes of the 3D reconstruction of the sample, recorded with UM at × 16 magnification (see movies to Fig. 7 in Supplementary material).
