Figure 1

Representative trace for substrate utilization inhibitor titration (SUIT) protocol for permeabilized muscle fibres; the oxygen concentration trace (dotted blue line) is on the left y-axis and respiration rate trace (black line) on the right y-axis. PMG pyruvate, malate, glutamate, D ADP, Succ succinate, Oligo oligomycin, ReO2 reoxygenation; substrate or drug concentrations are detailed in methods.