Figure 4
NF-κB target gene response to acute high shear stress—in vitro. Pro-inflammatory, NF-κB target genes were increased in ECs exposed to acute arterial WSS, in vitro. (C,D) HUVECs were exposed to AASS at 12 dyn/cm2 (A) and acute low shear stress (ALSS) at 0.5 dyn/cm2 (B) for 90 and 120 min in order to compare pro-inflammatory gene transcript levels by RT-qPCR. Data represent 6 independent experiments. (C,D) HUVECs were exposed to AASS at 12 dyn/cm2 for 4 h or maintained in static conditions, then immunostained for quantification of total CCL2 using CellProfiler. (C) Mean arbitrary fluorescence intensity of CCL2 staining was evaluated in whole cells. (D) Images are representative of 3 independent experiments and scale bar represents 15 µm. One-way ANOVA followed by post-hoc pairwise comparisons with Bonferroni correction (A,B) and two-tailed two-sample t-test (C) were used to calculate significance, where, * indicates p < 0.05.
