Figure 2
From: Northstar enables automatic classification of known and novel cell types from tumor samples
Annotation of glioblastoma and melanoma. (A) t-SNE of all cells in Darmanis et al. (2017) colored by original annotation20. (B) The number and type of cells that constitute the atlas data and the new glioblastoma data. (C) The same embedding of glioblastoma cells as in (A) but colored by northstar’s annotation and showing atlas cells (stars: atlas averages). (D) Sankey diagram with original manual annotations (left) vs. automated annotation by northstar (right). Band widths are proportional to the number of cells in the cluster. (E,F) Fraction of correctly assigned cells to known cell types (E) in the GBM dataset20 (mean of 3 northstar runs) and (F) in an independent study on melanoma in mice21 (mean of 5 runs) upon variation of the number of overdispersed features from the new dataset and the resolution parameter of the Leiden clustering. White stars indicate default parameters.
