Figure 6
From: Nitro-fatty acids suppress ischemic ventricular arrhythmias by preserving calcium homeostasis
CaMKII activity and RyR2 phosphorylation upon beta-adrenergic stimulation in isolated adult cardiomyocytes. (A) Immunoblot analyses after GST pulldown showing active (GST-HDAC4 419-670-bound) CaMKII in relation to total CaMKII (vehicle: 100% ± 36.1 vs. Iso: 116.5% ± 57.9 vs. Iso + NO2-OA: 66% ± 34.4). (B) Critical RyR2 phosphorylation under Iso stimulation and simultaneous NO2-OA treatment as indicated by immunoblotting (vehicle: 100% vs. Iso: 140% ± 22.9 vs. NO2-OA: 129% ± 19.8). (C) Phosphorylation of phospholamban (Thr17-p-PLN) in vehicle- vs. Iso- vs. Iso + NO2-OA treated cardiomyocytes (vehicle: 100% vs. Iso: 107.2% ± 0.1 vs. NO2-OA: 107.0% ± 2.9). n (cells out of 3 animals) = 9/9/9. Graphs show Mean ± SEM. Brackets indicate Mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001. The cropped blots are presented, and their full-length blots are included in the Supplemental Figures S4–S6.
