Table 2 Strains used in this study.

D39

Avery strain, clinical isolate, WT (capsular serotype 2), CSP1

^72,73

TIGR4

Invasive clinical isolate, WT (capsular serotype 4), CSP2

⁵³

8,655

Invasive isolate (serotype 6B), CSP2

CDC

3,875

Invasive isolate (serotype 6B), CSP1

CDC

∆spbhp-37 (SPZE1)

D39-derivative spbhp-37 null mutant, Ery^r

This study

E. coli

One shot Top-10

Cloning strain

Invitrogen

Plasmids

pAF104

Seamless cloning vector pUC19, Amp^r

This study

pCR2.1 TOPO

Cloning vector, Ery^r

⁶⁷