Table 3 Primers used in this study.
Target | Primers | Sequence (5′–3′) | Comments |
|---|---|---|---|
pUC19-L | ZE 738-L | TATCAAAGGGCATGCAAGCTTGGCGTAATCAT | Cloning |
ZE 739-R | ACTGTGCAGTACCGAGCTCGAATTCACTGGCC | ||
5′-region of spbhp 37 | ZE 740-L | CTCGGTACTGCACAGTAGTAGGTTTCCCTTTG | Cloning |
ZE 741-R | TTGTTCATTACTGAACCTCCTAAATAAGATGT | ||
ermB | ZE 742-L | GTTCAGTAATGAACAAAAATATAAAATATTCT | Cloning |
ZE 743-R | CATCAAGGCGACTCATAGAATTATTTCCTCCC | ||
3′-region of spbhp 37 | ZE 744-L | ATGAGTCGCCTTGATGGAAGCGTAAAAGTTCC | Cloning |
ZE 745-R | TGCATGCCCTTTGATAGACAAAACCACTTCTT | ||
aliB | ZE 878-L | GGACTGTTTCTCAGGACGGTTTG | qPCR |
ZE 879-R | CAGCTGCATATTGCAAACCTGTC | ||
ilvD | ZE 880-L | CCTGGTATGCGTTTCTCTCTAAC | qPCR |
ZE 881-R | AGCAATCATAGATCCAGGCATG | ||
aliA | ZE 882-L | GGTCACTTATGGGGATGAATGG | qPCR |
ZE 883-R | GGAATGTCACACCTTCTGCTTG | ||
argG | ZE 884-L | CCTTGGTATCTGCCTTGAGC | qPCR |
ZE 885-R | GATCCAAGGCTGCAATCGATAC | ||
SPD_1803 | ZE 886-L | GGATTGGATGAGGATTTCTACC | qPCR |
ZE 887-R | CTTCTCTAACAAGCCAAGACATG | ||
piuB | ZE 864-L | TGATTTCGACCAGCAGACCTG | qPCR |
ZE 865-R | CTGTACTCGGTGCAGCAAACTG | ||
rafE | ZE 964-L | GCTTGCTCCAACTATGGTAAATC | qPCR |
ZE 965-R | CATTGACGACTTTGACCTTGATC | ||
brnQ | ZE 966-L | CTCTATCTGGAGAACATTTTCTTCC | qPCR |
ZE 967-R | GCTATCTTCGTTGAAATCTCGTAG | ||
SPD_0740 | ZE 962-L | GTCATTGAGATGCGTGATATTACC | qPCR |
ZE 963-R | CATGTTCATTAGCGTGGACTTAC | ||
gyrB | ZE 661-L | GGCACTGTATGGTATCACACAAG | qPCR |
ZE 662-R | TCTCTAAATTGGGAGCGAATGTC |
