Figure 3
From: In vivo assessment of mechanisms underlying the neurovascular basis of postictal amnesia
Severe postictal hypoxia prevents LTP. (a) Recording paradigm. The right hemisphere was used for seizure induction and oxygen monitoring while the left hemisphere was used from electrophysiology of the PP-CA1 synapse. Responses were collected under urethane anesthesia from an acute electrode aimed at the stratum lacunosum moleculare of CA1. fEPSPs were evoked every 20 s and 3 consecutive responses were then averaged (one per minute). (b) Concurrent oxygen and electrophysiological recordings from rats that either went severely hypoxic or not after kindled seizures. Mean fEPSP slope ± SEM for each group is displayed before and after HFS. Inset shows representative evoked potentials from a rat in each group before (pre-injection, 10 min baseline) and after HFS (45–55 min post-HFS). (c) Quantification of B. Data from 45–55 min post-HFS was compared to the initial baseline (pre-injection). Two-way ANOVA revealed an effect of HFS (F(2,21) = 4.61, p = 0.02), group assignment (F(1,21) = 12.3, p = 0.02), and an interaction (F(2,21) = 4.61, p = 0.02). Tukey multiple comparisons are shown with significance levels (**p < 0.01, ***P < 0.001). (d) The postictal oxygen level at the time of LTP induction had a significant, positive correlation with the change in fEPSP slope.
