Figure 2
From: Alteration of gene expression in mice after glaucoma filtration surgery
q-PCR analysis of the selected genes. q-PCR analysis was carried out to validate microarray data using replicate cDNA samples. Significant upregulation of Il6, Sprr1b and downregulation of Gp2 gene expression were observed in both day 3 samples compared to the control. MMC treatment induced Krt1, Krt10, and Lor gene expression in bleb tissue and abolished filtration-induced Eln expression. MMC treatment suppressed Eln mRNA expression in mouse conjunctival fibroblasts and KRT1 and LOR mRNA expression in human conjunctival epithelial cells. Error bars indicate means ± s.d. (*P < 0.05, Welch’s t-test).
