Figure 10

qRT-PCR confirmation of EXOC7-H4-1 exocyst gene OE and RNAi through gene cassette expression in transgenic roots; EXOC7-H4-1 expression was induced by MAPKs but not observed within the syncytium, as determined in the DCM analyses. The results were compared to those in the RPS21 control employing the 2−ΔΔCT method31,50,69. A minimum cutoff of ± 1.5-fold was set, and p < 0.05. *Statistically significant. The p values for the replicate qRT-PCR analyses were calculated through Student’s t test70. Error bars represent the standard deviation. Please refer to the Materials and Methods (“Functional analysis of a MAPK-induced exocyst genes that were not expressed in the syncytium” section) for details of the analysis.