Figure 12 | Scientific Reports

Figure 12

From: Exocyst components promote an incompatible interaction between Glycine max (soybean) and Heterodera glycines (the soybean cyst nematode)

Figure 12The alternative text for this image may have been generated using AI.

Functional analyses demonstrated that genetic engineering of the candidate defense exocyst gene EXOC7-H4-1 had the expected effect on G. max defense against H. glycines. The calculated FIs for the OE and RNAi lines compared to the controls are shown with regard to the cysts per whole root (wr) system and per gram (pg) of root. The cyst count for OE analysis of the control (G. max[Williams 82/PI 518671]) was 203.09 ± 5.04 cysts per wr system and 49.03 ± 5.07 cysts pg of root. The cyst count for the RNAi analysis of the control (G. max[Peking/PI 548402]) was 9.94 ± 1.3 cysts per wr system and 2.52 ± 0.43 cysts pg of root. The values for the EXOC genes in their respective OE and RNAi analyses were statistically significant, as indicated in the figure. *Statistically significant, p < 0.05, as calculated by the Mann–Whitney–Wilcoxon (MWW) rank-sum test71. The experimental error representing the standard deviation is presented. The results are the average of three independently run biological replicates; all p values < 0.001. Please refer to the Materials and Methods section (“Functional analysis of a MAPK-induced exocyst genes that were not expressed in the syncytium” section) for details of the analysis.

Back to article page