Figure 3 | Scientific Reports

Figure 3

From: Exocyst components promote an incompatible interaction between Glycine max (soybean) and Heterodera glycines (the soybean cyst nematode)

Figure 3The alternative text for this image may have been generated using AI.

qRT-PCR confirmation of relative changes in transcript abundance of the exocyst subunit in the MAPK-OE and MAPK-RNAi transgenic roots first shown by RNA-seq analysis. In cases in which a numerical value of differential expression was obtained in the RNA-seq analyses (not necessarily ± 1.5-fold) and the p value was < 0.05 (p < 0.05), qRT-PCR was employed to confirm the relative change in transcript abundance of the exocyst gene of interest. These results were compared to the RPS21 control employing the 2−ΔΔCT method31,69,70. For a change in relative transcript abundance to be considered statistically significant, a minimum cutoff of ± 1.5-fold was set, and p < 0.05. The p values for the replicate qRT-PCR analyses were calculated through Student’s t test70. Error bars represent the standard deviation. Please refer to the Materials and Methods (“Functional analysis of a MAPK-induced exocyst genes that were not expressed in the syncytium” section) for details of the analysis.

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