Figure 4 | Scientific Reports

Figure 4

From: Exocyst components promote an incompatible interaction between Glycine max (soybean) and Heterodera glycines (the soybean cyst nematode)

Figure 4The alternative text for this image may have been generated using AI.

Analysis pipeline. (a) H. glycines-susceptible (S) G. max[Williams 82/PI 518671] (W82) (gray) was engineered to overexpress an exocyst component, as visualized by the reporter eGFP (green). Transgenic pRAP15-expressing control roots and roots overexpressing an exocyst gene were infected by H. glycines (magenta). After 30 days, the cysts (yellow) were extracted and enumerated with respect to the whole root (wr) system and cysts per gram (pg) of root system, resulting in a calculated female index (FI). (b) H. glycines-resistant (R) G. max[Peking/PI 548402] (Pek) roots were engineered to undergo RNAi of an exocyst component, as visualized by the reporter eGFP. Transgenic pRAP17-expressing control roots and roots undergoing RNAi targeting of the mRNA of an expressed exocyst gene were infected by H. glycines (magenta). After 30 days, the cysts were extracted and enumerated with respect to the wr system and cysts pg of root system, resulting in a calculated FI. Please refer to the Materials section (“Functional analysis of a MAPK-induced exocyst genes that were not expressed in the syncytium” section) for details.

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