Figure 8 | Scientific Reports

Figure 8

From: Exocyst components promote an incompatible interaction between Glycine max (soybean) and Heterodera glycines (the soybean cyst nematode)

Figure 8The alternative text for this image may have been generated using AI.

Functional analyses demonstrated that genetic engineering of the exocyst gene had the expected effect in the G. max root infected by H. glycines, as calculated by the FIs for the OE and RNAi lines compared to those of the controls with regard to the cysts per whole root (wr) system and per gram (pg) of root system. The cyst count for the OE analysis of the control (G. max[Williams 82/PI 518671]) was 203.09 ± 5.04 cysts per wr system and 49.03 ± 5.07 cysts pg of root system. The cyst count for RNAi analysis of the control (G. max[Peking/PI 548402]) was 9.94 ± 1.3 cysts per wr system and 2.52 ± 0.43 cysts pg of root system. The values for the EXOC genes in the respective OE and RNAi analyses were statistically significant, as indicated in the figure. *Statistically significant (p < 0.05), as calculated by the Mann–Whitney–Wilcoxon (MWW) rank-sum test71. The experimental error representing the standard deviation is presented. The results are the average of three independently run biological replicates; all p values < 0.001. Please refer to the Materials section (“Functional analysis of a MAPK-induced exocyst genes that were not expressed in the syncytium” section) for details of the analysis.

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