Figure 9

qRT-PCR confirmation of the relative change in EXOC7-H4-1 transcript abundance in MAPK-OE and MAPK-RNAi transgenic roots first analyzed by RNA-seq. In cases in which a numeric value indicating differential expression was obtained by the RNA-seq analyses and the p value was < 0.05 (p < 0.05), qRT-PCR is employed to confirm the relative change in transcript abundance. The results were compared to those in the RPS21 control employing the 2^−ΔΔCT method^31,50,69. For a change in relative transcript abundance to be considered statistically significant, a minimum cutoff of ± 1.5-fold was set, and p < 0.05. *Statistically significant. The p values for the replicate qRT-PCR analyses were calculated through Student’s t test⁷⁰. Error bars represent the standard deviation. Please refer to the Materials and Methods (“Functional analysis of a MAPK-induced exocyst genes that were not expressed in the syncytium” section) for details of the analysis.