Figure 9 | Scientific Reports

Figure 9

From: Exocyst components promote an incompatible interaction between Glycine max (soybean) and Heterodera glycines (the soybean cyst nematode)

Figure 9The alternative text for this image may have been generated using AI.

qRT-PCR confirmation of the relative change in EXOC7-H4-1 transcript abundance in MAPK-OE and MAPK-RNAi transgenic roots first analyzed by RNA-seq. In cases in which a numeric value indicating differential expression was obtained by the RNA-seq analyses and the p value was < 0.05 (p < 0.05), qRT-PCR is employed to confirm the relative change in transcript abundance. The results were compared to those in the RPS21 control employing the 2−ΔΔCT method31,50,69. For a change in relative transcript abundance to be considered statistically significant, a minimum cutoff of ± 1.5-fold was set, and p < 0.05. *Statistically significant. The p values for the replicate qRT-PCR analyses were calculated through Student’s t test70. Error bars represent the standard deviation. Please refer to the Materials and Methods (“Functional analysis of a MAPK-induced exocyst genes that were not expressed in the syncytium” section) for details of the analysis.

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