Figure 1 | Scientific Reports

Figure 1

From: Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal

Figure 1

Muscle fibre volumes of m. longissimus dorsi of ground squirrels. SA summer activity, HIB hibernation, IBA interbout arousal. Animals from the SA (n = 5), HIB (n = 5) and IBA (n = 4) groups were used. The volumes of 300–375 fragments of muscle fibres were calculated for each animal group (Supplementary Tables S8, S9). Image alignment was made using the IGL Align sEM Align program. The 3D images of muscle fibres were formed using IGL Trace software. Contours of muscle fibres were manually retouched in each image (Supplementary Figs. S1, S2). Quantitative parameters were calculated using 3D View 3.5 software. The statistical analysis of the results was carried out with SigmaPlot 11.0, from Systat Software, Inc., San Jose California USA, https://www.systatsoftware.com. The data were analysed using nonparametric single-factor dispersion analysis for repeated measurements (Kruskal–Wallis One Way Analysis of Variance on Ranks) followed with the pairwise comparison by the Tukey’s test. Values are means ± SEM (SD = 129.3 for SA, 119.9 for HIB and 113.3 for IBA). **p < 0.01 (vs SA), ##p < 0.01 (IBA vs HIB).

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