Figure 1
Schematic representation of the process of cfNA sampling from clinical specimens using a complex of amine-modified diatomaceous earth (DE) coated by cucurbit[6]uril (CB) (the biocomposite platform). First, the biocomposite platform was prepared for ready-to-use cfNA sampling. The biocomposites were added to blood plasma samples and incubated at RT for 10 min with shaking for 30 s at 2 min intervals for cfNAs capture. The solution was centrifuged to remove the supernatant containing debris, and the precipitate was washed three times with 1 mL PBS. Finally, high pH (pH 10.6) elution buffer was added and centrifuged to extract cfNAs. The biocomposite platform was able to isolate cfNAs from clinical specimens at high concentrations and purity within 20 min without requiring large equipment or a thermo-regulator.
