Table 1 Immune cell phenotypic markers.

From: The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells

Cell type

Positive markers

Negative markers

Immune cell

CD45 +, Live, Singlets

DAPI +, Doublets

B Cell (adaptive immune cell)

CD45 + B220 + 

CD3e−, CD11b−

T Cell (adaptive immune cell)

CD45 + CD3e + 

B220− CD11b−

Natural killer (NK) cell

CD45 + NK1.1 + 

CD3e−

Myeloid cell (innate immune cell)

CD45 + CD11b + 

B220− CD3e−

Cochlear macrophage

CD45 + CD11b + CX3CR1 + 

 

Neutrophil

CD45 + CD11b + Ly6G + 

CD11c−

Circulating monocyte

CD45 + CD11b + Ly6G + CD11c + 

 
  1. Surface markers CD45, B220+, CD3e, NK1.1, CD11b, CX3CR1, and Ly6G were used to sort immune cells, B cells, T cells, NK cells, myeloid cells, macrophages, and neutrophils respectively, by flow cytometry. The detailed strategy has been discussed in the methods section. Table shows the positive and negative markers used to sort the immune cells by flow cytometry.
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