Figure 4
Incorporation of HMC3 human microglia into hBORGs. HMC3 microglia (1 × 106 cells) were infected with HIV-1 (green) or mock-infected, membrane-labeled (red) and were added to hBORGs labeled with Hoechst-stained (blue). After 24 h, microglia incorporated-hBORGs were transferred to a new plate and maintained for an additional 15 days for further analyses. White arrowheads point to HIV-infected microglia (green) in insert a and b, and White asterisks in insert a and b point to uninfected microglia (red). Scale bar is 200 μm and 50 μm in inserts.
