Figure 6 | Scientific Reports

Figure 6

From: Modeling HIV-1 neuropathogenesis using three-dimensional human brain organoids (hBORGs) with HIV-1 infected microglia

Figure 6

Characterization of viability and cytotoxicity induced by HIV-1 in primary MG-hBORG model. (A) Mock-infected and HIV-infected MG-hBORGs were stained with LIVE/DEAD Cell imaging kit to determine the live and dead cells on days 3 and 8 post microglia incorporation. Calcein stains live cells (green) and ethidium homodimer stains nuclei of dead cells (red). Scale bars is 100 μm. (B) Calcein and ethidium homodimer positive cells were counted on day 8 and the corresponding ratio of live (calcein +) and dead (EthH +) cells were calculated. (C) Cytotoxicity induced by HIV infection was quantified in the supernatant of the HIV-infected, mock-infected MG-hBORGs and hBORGs by measuring LDH activity and % toxicity was determined (N = 3).

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