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Figure 1

From: The dynamics between in vitro culture and metabolism: embryonic adaptation to environmental changes

Figure 1

After in vitro maturation and fertilization, the presumed zygotes were randomly distributed for in vitro culture at 20 or 5% of oxygen and remained in the same oxygen tension for the total time of culture. At 40 h post insemination (hpi), the embryos were again assessed, classified and separated as Fast (≥ 4 cells) or Slow (2 or 3 cells). About 98 hpi, the embryos of each group were again randomly transferred to 3 glucose treatments (0.6, 2 or 5 mM) resulting in a total of 12 study groups.

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