Figure 3
From: Velvet activated McrA plays a key role in cellular and metabolic development in Aspergillus nidulans
The effects of ΔmcrA on production of secondary metabolites. (A) Thin-layer chromatography (TLC) of secondary metabolites from WT, ΔmcrA (TMK19), and C’mcrA (TMK20) strains under dark for 3 days stationary culture with mycelia. (B) Amount of ST in conidia of WT, ΔmcrA (TMK19), and C’mcrA (TMK20) strains by HPLC analysis (measured in triplicates, ***p < 0.001). (C) Secondary metabolites in asexual spores by HPLC analysis. Asexual spores (2Χ108) of WT, ΔmcrA (TMK19), and C’mcrA (TMK20) strains were collected and extracted with chloroform, processed, and subjected to HPLC analysis. The blue arrow designates ST. The green arrow designates unknown metabolites (measured in triplicates). (D) Amount of ST in conidia of WT, ΔmcrA (TMK19), and C’mcrA (TMK20) conidia (measured in duplicate, ***p < 0.001). (E) Amount of unknown metabolite in conidia of WT, ΔmcrA (TMK19), and C’mcrA (TMK20) strains (measured in duplicate, *p < 0.05). (F) Levels of aflR mRNA in the WT, ΔmcrA, and C’mcrA conidia.
