Figure 4

Overexpression of mcrA causes pleiotropic effects. (A) Amount of ST production in WT (FGSC4) and OEmcrA (THS41; alcA(p)::mcrA) strains. The supernatant of each strain following 3 days of stationary culture was extracted using chloroform and subjected to TLC analysis. (B) WT (FGSC4) and OEmcrA (THS41; alcA(p)::mcrA) strains were point inoculated onto solid MMG (non-inducing) or onto solid MMT (inducing) including 0.5% YE medium. Photographs of the cultures at day 5 are also shown. The bottom panels indicate close-up views of the middle of the plates (bar = 100 μm). (C) Quantitative analysis of conidiospore formation of the strains shown in (B). The numbers of conidia per plate were counted in triplicates (**p < 0.01; ***p < 0.001). (D) Relative AB reduction rates of WT and OEmcrA (THS41) strains grown under submerged culture conditions at 37 °C. The percent of alamarBlue (AB) reduction represents the fungal cell viability. The mean values were represented by a bar graph.