Table 2 Oligonucleotides used in this study.
From: Velvet activated McrA plays a key role in cellular and metabolic development in Aspergillus nidulans
Name | Sequence (5′ 3′) | Purpose |
|---|---|---|
OHS767 | TCGAAGAGTTTGCCCCACAGC | 5′ flanking of mcrA |
OHS769 | GGCTTTGGCCTGTATCATGACTTCA CATTGGAAGGTCGGGGAGCAG | 3′ mcrA with AfupyrG tail |
OHS770 | TTTGGTGACGACAATACCTCCCGAC CCATCTTCAATGCCCAATATGCTC | 5′ mcrA with AfupyrG tail |
OHS768 | AGCACTGTGGATGACAGCTCAAC | 3′ flanking of mcrA |
OHS771 | CGACCCCAACTCTACCAGGACTC | 5′ nest of mcrA |
OHS772 | CAATCGCTCTAACTGTCTACTCGCG | 3′ nest of mcrA |
OMK607 | TCACATCTCGATGATTGGTTGAATG | 5′ flanking of flbA |
OMK613 | GCTTTGGCCTGTATCATGACTTCA TGGCATTGAAGAGTGCAGGTCGGAG | 3′ flbA with AfupyrG tail |
OMK614 | ATCGACCGAACCTAGGTAGGGTA ACAGTAATTATCTACACGCGTGATG | 5′ flbA with AfupyrG tail |
OMK610 | ACTACTCACTACCTAACTTGACTG | 3′ flanking of flbA |
OMK611 | TGGTTGAATGGTGTATGGGTCAGC | 5′ nest of flbA |
OMK612 | TGTAGCTTTCGTTCAGGCGATAGTG | 3′ nest of flbA |
OMK608 | ACTTCTGCAGTCGGAATTGGCCTG TGGCATTGAAGAGTGCAGGTCGGAG | 3′ flbA with AnipyroA tail |
OMK609 | TGGTGAGAACACATGCACAACTTG ACAGTAATTATCTACACGCGTGATG | 5′ flbA with AnipyroA tail |
OHS875 | ATATGAATTCATGTCGAACAATCCGAACCCG | 5′ mcrA_EcoRI |
OHS878 | ATATGCGGCCGCCTGACCCAATCCACGGCGGT | 3′ mcrA _NotI |
OHS657 | ATATGAATTCGGTGTAATTCTGGGTGTCTTGG | 5′ mcrA for C’_EcoRI |
OMK589 | GCTGAAGTCATGATACAGGCCAAA | 5′ AfupyrG marker |
OMK590 | ATCGTCGGGAGGTATTGTCGTCAC | 3′ AfupyrG marker |
ONK395 | ATCTCATGGGTGCTGTGCGAAAGG | 5′ AnipyroA marker |
ONK396 | TTGCATCGCATAGCATTGCATTGC | 3′ AnipyroA marker |
OMK578 | CTGGCAGGTGAACAAGTC | 5′ brlA probe |
OMK579 | AGAAGTTAACACCGTAGA | 3′ brlA probe |
OHS0044 | GTAAGGATCTGTACGGCAAC | 5′ actin RT_probe |
OHS0045 | AGATCCACATCTGTTGGAAG | 3′ actin RT_probe |
OHS0578 | TGAGGAATTGACCACCGACA | 5′ fksA RT_probe |
OHS0579 | GCACCAAGGATAGCAACAGG | 5′ fksA RT_probe |
OHS0599 | GCGCGAAGAAGACTTCAAC | 5′ aflR RT_probe |
OHS0600 | TGCAATAACTGCCGACGAC | 3′ aflR RT_probe |
