Table 1 List of oligonucleotide sequences and their PCR conditions used in the current study.
Target gene | Primer sequences 5′-3' | Amplicon (bp) | PCR conditions | References | ||||
|---|---|---|---|---|---|---|---|---|
No of cycles | Denaturation | Annealing | Extension | Final Extension | ||||
oprL | ATG GAA ATG CTG AAA TTC GGC CTT CTT CAG CTC GAC GCG ACG | 504 | 40 | 96 ˚C for 1 min | 55 ˚C for 1 min | 72 ˚C for 1 min | 72˚C for 10 min | |
toxA | GACAACGCCCTCAGCATCACCAGC CGCTGGCCCATTCGCTCCAGCGCT | 396 | 30 | 94 ˚C for 1 min | 55 ˚C for 1 min | 72 ˚C for 1 min | ||
exoS | GCGAGGTCAGCAGAGTATCG TTCGGCGTCACTGTGGATGC | 118 | 36 | 94 ˚C for 30 s | 58 ˚C for 30 s | 68 ˚C for 1 min | ||
phzM | ATGGAGAGCGGGATCGACAG ATGCGGGTTTCCATCGGCAG | 875 | 30 | 94 °C for 30 s | 54 ˚C for 30 s | 72 ˚C for 1 min | ||
blaTEM | ATCAGCAATAAACCAGC CCCCGAAGAACGTTTTC | 516 | 32 | 94 °C for 30 s | 54 ˚C for 30 s | 72 ˚C for 1 min | ||
tetA | GGTTCACTCGAACGACGTCA CTGTCCGACAAGTTGCATGA | 576 | 30 | 94 ˚C for 1 min | 56 ˚C for 30 s | 72 ˚C for 1 min | ||
blaCTX-M | ATGTGCAGYACCAGTAARGT TGGGTRAARTARGTSACCAGA | 593 | 35 | 95 ˚C for 45 s | 51 ˚C for 45 s | 72 ˚C for 1 min | ||
