Figure 3 | Scientific Reports

Figure 3

From: Hepatitis C virus enhances Rubicon expression, leading to autophagy inhibition and intracellular innate immune activation

Figure 3

Late-stage autophagy inhibition activated innate immunity. (A, B) HCV replicon-harbouring cells were treated with 125 nM bafilomycin A1 for the indicated durations. Western blot analysis of HCV NS5A and LC3 (A). The mRNA expression levels of type 1 interferon-related genes and HCV RNA levels in cells (B). (C, D) HCV replicon-harbouring cells were transfected with Crispr/Cas9 plasmids against Atg7 or a negative control plasmid (Cas9). Western blot analysis of ATG7, HCV NS5A and LC3 (C). Western blot analysis of HCV NS5A and LC3 (C). The mRNA expression levels of type 1 interferon-related genes and HCV RNA levels in cells (D). (E) The mRNA expression levels of interferon-stimulated genes (ISGs) in HCV replicon-harbouring cells transfected with GFP plasmid or GFP-Rubicon plasmid for 72 h (left panel), treated with or without 125 nM bafilomycin A1 for 24 h (middle panel), and transfected with Crispr/Cas9 plasmid against Atg7 (siAtg7) or a negative control plasmid (Cas9) (right panel). *p < 0.05.

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