Figure 3 | Scientific Reports

Figure 3

From: Ocular surface inflammation induces de novo expression of substance P in the trigeminal primary afferents with large cell bodies

Figure 3

Immunolocalization of substance P in the corneal intraepithelial nerve terminals. The length of intraepithelial nerve terminals stained for substance P (SP) and βш-tubulin, a pan-neuronal marker, was measured and the SP-immunoreactivity (IR) (%) of corneal intraepithelial nerve terminals was calculated using Image J software. SP-IR (%) of corneal intraepithelial nerve terminals did not show any difference among the groups. In the BAK and 1 w groups, representing the acute phase of inflammation, SP-IR or background intensity was also observed in the neighbouring cells and extracellular matrix besides the nerve fibres. Scale bar = 20 μm.

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