Figure 5 | Scientific Reports

Figure 5

From: Early-stage knee OA induced by MIA and MMT compared in the murine model via histological and topographical approaches

Figure 5

Segmentation of the polarized-light red sirius slices. Segmentation is obtained from white-light pictures, which offer better results and easier handling than polarized light pictures. The slice is placed under the lens of the microscope. Picture is taken with (a) and without polarized filter (d). Cartilage semi-automatic segmentation is performed on white-light picture (b). A mask is made from this segmentation (b) and is applied with AND operator (e). Segmented polarized cartilage is obtained (f) and is used for quantification. All these operations were done on the ImageJ software (ImageJ 1.53c, Wayne Rasband, NIH USA, http://imagej.nih.gov/ij). Scale bar = 0.25 mm.

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