Figure 1 | Scientific Reports

Figure 1

From: Regulation of Ras homolog family member G by microRNA-124 regulates proliferation and migration of human retinal pigment epithelial cells

Figure 1

Analysis of miR-124 and RhoG expression during cell proliferation. (A) Representative images of cell confluence after plating 7.5 × 104 RPE cells/mL in 6-well cell culture plates. (B) At the indicated time points, RPE cells were counted in triplicate cell culture wells. (C) Quantitative measurements of endogenous miR-124 expression levels in RPE cells at the indicated time points. The quantitative miRNA expression results were normalized to RNU6 and miR-16 expression levels. (D) The protein levels of cell cycle regulatory factors and RhoG were analyzed by western blotting. (E) Quantitative evaluation of endogenous miR-124 expression in non-treated or miR-124 inhibitor-treated RPE cells. (F) Expression of RhoG in non-treated or miR-124 inhibitor-treated RPE cells was assessed using western blotting.

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